Tissue homogenization as a key step in extracting RNA from human and rat pancreatic tissue.

نویسندگان

  • G Sparmann
  • A Jäschke
  • M Loehr
  • S Liebe
  • J Emmrich
چکیده

1.Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson. 1989. Molecular Biology of the Cell, 2nd ed. Garland Publishing, New York. 2.Atlas, R.M. and L.C. Parks. 1993. Handbook of Microbiological Media, p. 766-767. CRC Press, Boca Raton. 3.Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159. 4.Gopalakrishna, Y., D. Langley and N. Sarkar. 1981. Detection of high levels of polyadenylate-containing RNA in bacteria by the use of a single-step RNA isolation procedure. Nucleic Acids Res. 9:3545-3554. 5.Kikuta-Oshima, L.C., F.D. Quinn, W.R. Butler, T.M. Shinnick and C.H. King. 1995. Isolation of RNA from Mycobacterium tuberculosis using a nitrogen decompression chamber. BioTechniques 18:987-990. 6.Majumdar, D., Y.J. Avissar and J.H. Wyche. 1991. Simultaneous and rapid isolation of bacterial and eukaryotic DNA and RNA: a new approach for isolating DNA. BioTechniques 11:94-101. 7.Reddy, K.J., R. Webb and L.A. Sherman. 1990. Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge. BioTechniques 8:250-251. 8.Schmitt, M.E., T.A. Brown and B.L. Trumpower. 1990. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18:3091-3092. 9.Usui, M. and M. Yamaguchi. 1995. Extraction of nucleic acid using agglutination partition method. Igakunoayumi 173:947-950. 10.Utt, E.A., J.P. Brousal, L.C. Kikuta-Oshima and F.D. Quinn. 1995. The identification of bacterial gene expression differences using mRNA-based isothermal subtractive hybridization. Can. J. Microbiol. 41:152-156.

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عنوان ژورنال:
  • BioTechniques

دوره 22 3  شماره 

صفحات  -

تاریخ انتشار 1997